publications

For full list, please see Google Scholar

2026

Samantha Bratcher, Michael R. E. Lamont, Chris B. Schaffer, Karl J. Lewis. Depth-Dependent Advantages of Three-Photon Microscopy for Imaging Through Intact Murine Cortical Bone and Into the Marrow. bioRxiv 2026.07.31.742087; doi: https://doi.org/10.64898/2026.07.31.742087

Melia D. Matthews, Katelyn Lunny, Lakhi Raju, Nada Naguib, Saniya Tariq, Ulirich B. Wiesner, Karl J. Lewis. Intravital Depletion of Cholesterol Reduces Membrane Dynamics and Increases Mechanosensitivity in Osteocytes In Vivo. bioRxiv 2026.07.21.739862; doi: https://doi.org/10.64898/2026.07.21.739862

Samantha Bratcher, Macy Mora-Antoinette, Karl J. Lewis. Real-Time Axial Motion Compensation for Intravital Two-Photon Imaging of Mechanically Loaded Bone. bioRxiv 2026.07.17.739194; doi: https://doi.org/10.64898/2026.07.17.739194

2025

Wasi, M., Lewis, K.J. Intravital Microscopy of Osteocytes: A New Era in Bone Cell Biology. Curr Osteoporos Rep 23, 50 (2025). https://doi.org/10.1007/s11914-025-00942-1

Matthews, M.D., Saffari, A., Mukul, N. et al. Real-time visualization and modulation of endocytic dynamics in osteocytes in vivo. Sci Rep 15, 31454 (2025). https://doi.org/10.1038/s41598-025-05735-1

D Laudier, M Mora-Antionette, A Garcia-Ortiz, KJ Lewis, Transparent Bones: An Improved BABB Protocol for Clearing and 3D Imaging of Intact Calcified Tissues DOI: https://dx.doi.org/10.17504/protocols.io.3byl4zk2ovo5/v1

Macy Mora-Antoinette et al., Cholinergic regulation of osteocyte mechanobiology: A paradigm for bone adaptation.Sci. Adv.11,eads9720(2025).DOI:10.1126/sciadv.ads9720

Matthews, M.D., Saffari, A., Mukul, N. et al. Real-time visualization and modulation of endocytic dynamics in osteocytes in vivo. Sci Rep15, 31454 (2025). https://doi.org/10.1038/s41598-025-05735-1

2023

MD Matthews, E Cook, N Naguib, UB Wiesner, KJ Lewis, “Intravital imaging of osteocyte integrin dynamics with locally injectable fluorescent nanoparticles,” Bone (2023)

2021

KJ Lewis, “Osteocyte calcium signaling – A potential translator of mechanical load to mechanobiology,” Bone (2021)

KJ Lewis, PC Zuckerman, JF Boorman-Padgett, J Basta-Pljakic, J Louie, S Stephen, DC Spray, MM Thi, Z Seref-Ferlengez, RJ Majeska, S Weinbaum, MB Schaffler, “Estrogen depletion on In vivo osteocyte calcium signaling responses to mechanical loading,” Bone (2021)

2020

KJ Lewis, X Yi, CS Wright, EZ Pemberton, WA Bullock, WR Thompson, AG Robling. “The mTORC2 Component Rictor Is Required for Load-Induced Bone Formation in Late-Stage Skeletal Cells,” JBMR Plus (2020)

2019

KJ Lewis, RB-J Choi, EZ Pemberton, WA Bullock, AB Firulli, AG Robling, “Twist1 inactivation in Dmp1-expressing cells increases bone mass but does not affect the anabolic response to sclerostin neutralization,” ‎Int. J. Mol. Sci (2019)

WA Bullock, AM Hoggatt, DJ Horan, KJ Lewis, H Yokota, S Hann, ML Warman, A Sebastian, GG Loots, FM Pavalko, AG Robling, “Expression of a Degradation‐Resistant β‐Catenin Mutant in Osteocytes Protects the Skeleton From Mechanodeprivation‐Induced Bone Wasting,” JBMR (2019)

2017

KJ Lewis, D Frikha-Benayed, J Louie, S Stephen, DC Spray, MM Thi, Z Seref-Ferlengez, RJ Majeska, S Weinbaum, MB Schaffler, “Osteocyte calcium signals encode strain magnitude and loading frequency in vivo,” PNAS 114(44):11775-11780 (2017)

2014

C McGoverin, KJ Lewis, M Bostrom, and N Pleshko, "The Contribution of Bone and Cartilage to the Near-Infrared Spectrum of Osteochondral Tissue," Appl. Spectrosc.  68, 1168-1175 (2014)